期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 393, 期 2, 页码 259-263出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.01.115
关键词
DDA3; Phosphorylation; Spindle; Chromosome congression; Mitosis
资金
- Korea Research Foundation of the Korean Government (MOEHRD) [KRF-2006-352-C00057]
- Burroughs-Wellcome Career Award in Biomedical Research
- National Institutes of Health [CM062852, HL079442, RR11823-10]
DDA3 is a microtubule-associated protein that controls chromosome congression and segregation by regulating the mitotic spindle. Depletion of DDA3 alters spindle structure, generates unaligned chromosomes at metaphase, and delays the mitotic progression. Through a mass spectrometry analysis, we found that DDA3 is phosphorylated on Ser225 during mitosis. Phosphorylation of this residue is important for the mitotic function of DDA3, as the phospho-mimicking DDA3-S225D variant, but not the nonphosphorable DDA3-S225A mutant, rescues the DDA3-knockdown phenotype. We conclude that the mitotic function of DDA3 is regulated by phosphorylation on the Ser225 residue. (C) 2010 Elsevier Inc. All rights reserved.
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