期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 67, 期 7, 页码 3304-3308出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.67.7.3304-3308.2001
关键词
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Pseudomonas stutzeri OX1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. Phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethgl-phenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, however, were not cleaved by catechol 2,3-dioxygenase, The identified gene cluster displayed a gene order similar to that of the Pseudomonas sp, strain CF600 dmp operon for phenol catabolism and was found to be coregulated by the tou operon activator TouR A hypothesis about the evolution of the toluene and o-xylene catabolic pathway in P. stutzeri OX1 is discussed.
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