期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 398, 期 3, 页码 444-449出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.06.096
关键词
Human serum albumin; Flavonoid binding; Daidzein; Daidzein metabolites; Genistein; Naringenin; Quercetin; Oleate; Thermodynamics; Allostery
资金
- Ministero dell'Istruzione, dell'Universita e della Ricerca of Italy [CLAR 2009]
- Ministero della Salute of Italy
- Grants-in-Aid for Scientific Research [22650231] Funding Source: KAKEN
Dietary flavonoid may have beneficial effects in the prevention of chronic diseases. However, flavonoid bioavailability is often poor probably due to their interaction with plasma proteins. Here, the affinity of daidzein and daidzein metabolites as well as of genistein, naringenin, and quercetin for human serum albumin (HSA) has been assessed in the absence and presence of oleate. Values of the dissociation equilibrium constant (K) for binding of flavonoids and related metabolites to Sudlow's site I range between 3.3 x 10(-6) and 3.9 x 10(-5) M, at pH 7.0 and 20.0 degrees C, indicating that these flavonoids are mainly bound to HSA in vivo. Values of K increase (i.e., the flavonoid affinity decreases) in the presence of saturating amounts of oleate by about two folds. Present data indicate a novel role of fatty acids as allosteric inhibitors of flavonoid bioavailability, and appear to be relevant in rationalizing the interference between dietary compounds, food supplements, and drugs. (C) 2010 Elsevier Inc. All rights reserved.
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