期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 401, 期 4, 页码 538-543出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.09.089
关键词
FoF1 ATP synthase; ATP synthesis; Mitochondria; High-throughput analysis; Streptolysin
A new assay has been developed to measure mitochondrial ATP synthesis of cultured mammalian cells Cells in a microplate are exposed to streptolysin O to make plasma membranes permeable without damaging mitochondrial function and ATP synthesis is monitored by luciferase Addition of inhibitors of FoF1-ATP synthase (FoF1) respiratory chain TCA cycle and ATP/ADP translocator as well as knockdown of beta-subunit of FoF(1) resulted in loss of ATP synthesis Compared with the conventional procedures that need mitochondria fractionation and detergent this assay is simple sensitive and suitable for high-throughput analysis of genes and drugs that could affect mitochondrial functional integrity as represented by ATP synthesis activity (C) 2010 Elsevier Inc All rights reserved
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