期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 401, 期 1, 页码 64-68出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2010.09.009
关键词
Cytochrome c oxidase; Complex IV subunits; Electron transport chain; Mitochondria; Aging; Oxidative stress
资金
- National Institutes of Health-National Institute on Aging [RO1 AG7657]
The hypothesis, that structural deterioration of cytochrome c oxidase (CcO) is a causal factor in the agerelated decline in mitochondrial respiratory activity and an increase in H2O2 generation, was tested in Drosophila melanogaster. CcO activity and the levels of seven different nuclear DNA-encoded CcO subunits were determined at three different stages of adult life, namely, young-, middle-, and old-age. CcO activity declined progressively with age by 33%. Western blot analysis, using antibodies specific to Drosophila CcO subunits IV, Va, Vb, VIb, VIc, VIIc, and VIII, indicated that the abundance these polypeptides decreased, ranging from 11% to 40%, during aging. These and previous results suggest that CcO is a specific intra-mitochondrial site of age-related deterioration, which may have a broad impact on mitochondrial physiology. (C) 2010 Elsevier Inc. All rights reserved.
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