F0F1-ATPase activity regulated by external links on beta subunits

F0F1-ATPase activity is regulated by external links on beta subunits with different molecular weight. It is inhibited when anti-beta-subunit antibody, streptavidin and H9 antibody link on the beta subunits successively. but is activated when virus was binded Western blotting indicated that the employed anti-beta antibody target was on the non-catalytic site of the beta subunit. Furthermore, an ESR study of spin-labeled ATP (SL-ATP) showed that the affinity of ATP to the holoenzyme increases with increasing external links on the beta Subunits This simple regulation method may have great potential in the design of rapid free, labeled sensitive and selective biosensors (c) 2009 Elsevier Inc All rights reserved