期刊
PHYTOCHEMICAL ANALYSIS
卷 12, 期 4, 页码 226-242出版社
WILEY
DOI: 10.1002/pca.589
关键词
reverse phase high performance liquid-chromatography; nuclear magnetic resonance spectroscopy; mass spectrometry; chemical synthesis; extraction; glucosinolates; desulphoglucosinolates
Methods are described for the optimised extraction, desulphation and HPLC separation of desulphoglucosinolates, These methods provide rapid separation, identification and quantitative measurements of glucosinolates extracted from Brassica napus L and related crops, of unusual glucosinolates found in crucifer weed species, and also of synthetic alkylglucosinolates, The desulphoglucosinolates used in these studies were either chemically synthesised (at least one example from each major structural class), or purified from various plant sources. Validation of the identities of the desulphoglucosinolates was by comparison of retention times with standards, and by UV,H-1- and C-13-NMR and chemical ionisation MS analysis. A list of useful species, and the specific tissues, from which high concentrations of standards can be extracted is included. Copyright (C) 2001 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据