期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 391, 期 1, 页码 407-413出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.11.071
关键词
Post-translational modification; SUMO; DNA replication; Chromatin; CAF-1
资金
- Naito Foundation
- Novartis Foundation
- Ministry of Education, Culture, Sport, Science and Technology (MEXT)
- Japan Society for the Promotion of Science (JSPS)
The small ubiquitin-related modifier 2/3 (SUMO2/3) can be post-translationally conjugated to a wide variety of proteins constituting chromatin, the platform for genetic and epigenetic regulation Nevertheless, it IS unclear how SUMO2/3 and SUMO2/3-modified proteins are delivered to the chromatin fibers Here we report that the largest subunit of chromatin assembly factor 1 (CAF-1), human p150, interacts directly and preferentially with SUMO2/3. Amino acid residue of 98-105 in p150 is essential and sufficient for SUMO2/3 interaction p150-SUMO2/3 interaction coincided with regions that replicate chromatin fibers, because accumulation of the proliferating cell nuclear antigen (PCNA), and incorporation of bromodeoxyuridine (BrdU) were detected at foci co-localized with both p150 and SUMO2/3 during the S-phase in a cell line expressing epitope-tagged p150 Although inhibition of SUMO2/3 expression had only a small effect on p150 deposition on the replication sites, depletion of p150 led to delocalization of SUMO2/3 from the replication foci Furthermore. p150 mutants deficient in SUMO2/3 interaction. Caused a major reduction of SUMO2/3 at the replication foci Thus. Our findings Suggest an expanded role of p150 as a SUMO2/3-interacting factor, and raise the intriguing possibility that p150 plays a role in promoting delivery of SUMO2/3 or SUMO2/3-modified proteins (or both) on chromatin fibers during replication. (C) 2009 Elsevier Inc All rights reserved.
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