High-resolution NMR-based metabolic detection of microgram biopsies using a 1 mm HRμMAS probe

A prototype 1 mm High-Resolution micro-Magic Angle Spinning (HR mu MAS) probe is described. High quality H-1 NMR spectra were obtained from 490 mu g of heterogeneous biospecimens, offering a rich-metabolite profiling. The results demonstrate the potential of HR mu MAS as a new NMR analytical tool in metabolomics.