期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 379, 期 2, 页码 456-459出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.12.078
关键词
beta-1,4-Galactosyltransferase V; Knockout mice; Growth retardation; Embryonic lethality; N-glycans; O-glycans; Lactosylceramide
资金
- Ministry of Education, Science, Culture and Sports of Japan [109240104, 12680708]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [12680708] Funding Source: KAKEN
The beta-1,4-galactosyltransferase (beta-1,4-GalT) V whose human and mouse genes were cloned by us has been suggested to be involved in the biosynthesis of N-glycans and O-glycans, and lactosylceramide. To determine its biological function, beta-1,4-GalT V (B4galt5) mutant mice obtained by a gene trap method were analyzed. Analysis of pre- and post-implantation embryos revealed that the B4galt5(-/-) mice die by El 0.5 while B4galt5(+/-) mice were born and grown normally. Histological study showed that most tissues are formed in B4galt5(-/-) embryos but their appearance at Ell 0.5 is close to that of B4galt5(-/-) embryos at E9.0-9.5. The results indicate that the growth is delayed by one to one and half day in B4galt5(-/-) embryos when compared to B4galt.5'1- embryos, which results in early death of the embryos by E10.5, probably due to hematopoietic and/or placental defects. (C) 2008 Elsevier Inc. All rights reserved.
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