期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 381, 期 3, 页码 322-327出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.01.174
关键词
Mesenchymal stem cells; Adipogenic differentiation; Cell density; Micropattern; Tissue engineering
资金
- World Premier International Research Center (WPI) Initiative on Materials Nanoarchitectonics
- MEXT, Japan
- New Energy and Industrial Technology Development Organization of Japan
The effect of cell density on the adipogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) was investigated by using a patterning technique to induce the formation of a cell density gradient on a micropatterned Surface. The adipogenic differentiation of MSCs at a density gradient from 5 x 10(3) to 3 x 10(4) cells/cm(2) was examined. Lipid vacuoles were observed at all cell densities after 1-3 weeks of culture in adipogenic differentiation medium although the lipid vacuoles were scarce at the low cell density and abundant at the high cell density. Real-time RT-PCR analysis showed that adipogenesis marker genes encoding peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2), lipoprotein lipase (LPL), and fatty acid binding protein-4 (FABP4) were detected in the MSCs cultured at all cell densities. The results suggest that there was no apparent effect of cell density on the adipogenic differentiation of human MSCs. (C) 2009 Elsevier Inc. All rights reserved.
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