4.3 Article

Extraction and purification of DNA in rhizosphere soil samples for PCR-DGGE analysis of bacterial consortia

期刊

JOURNAL OF MICROBIOLOGICAL METHODS
卷 45, 期 3, 页码 155-165

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(01)00253-6

关键词

DNA extraction; DNA purification; DGGE; microbial community analysis; PCR; rhizosphere; soil

向作者/读者索取更多资源

Application of DNA fingerprinting methods enables the detection of diverse members of soil bacterial consortia, even including those bacteria not yet cultivated. However, extraction and purification of DNA from soil samples without bias is difficult. We compared five different DNA isolation methods and three purification methods for rhizosphere soil samples. Purified DNA extracts were amplified in PCR using universal bacterial primers and the PCR products were analysed with denaturing gradient gel electrophoresis (DGGE) for the visualisation of DNA bands representing dominant bacterial species. Both the isolation and purification methods affected the apparent bacterial community structure of the samples. (C) 2001 Elsevier Science B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据