期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 390, 期 4, 页码 1389-1394出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.10.165
关键词
Calcium binding proteins; Sarcoplasmic reticulum; Cardiac muscle; Excitation-contraction coupling
资金
- Korea Ministry of Science and Technology [M1050301001-6N0301-0110]
- GIST
- KISTI-KREONET
Junctate is a newly identified sarcoplasmic reticulum (SR) Ca2+ binding protein, but its function in cardiac muscle has remained unclear. Our previous study showed that chronic over-expression of junctate in transgenic mice led to altered SR functions and development of severe hypertrophy. in this study, we identified the interaction of junctate with SERCA2a by co-immunoprecipitation and GST-pull-down assay. This interaction was inhibited by higher Ca2+ concentration. Immunolocalization assays also showed that junctate and SERCA2a were co-localized in the SR of cardiomyocytes. Direct binding of the C-terminal region of junctate (amino acids 79-270) and luminal domain of SERCA2a (amino acids 70-89) was observed by deletion mutation experiments. Adenovirus-mediated transient over-expression of junctate in cardiomyocytes showed a reduced decay time of Ca2+ transients and increased oxalate-supported SERCA2 Ca2+ uptake, suggesting an increased activity of SERCA2a. Taken together, according to our data, junctate may play an important role in the regulation of SR Ca2+ cycling through the interaction with SERCA2a in the murine heart. (C) 2009 Elsevier Inc. All rights reserved.
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