期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 390, 期 2, 页码 192-195出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2009.08.164
关键词
Heterochiral; DNA; EcoRI-promoted hydrolysis; Kinetics; FRET
We found unusual reactivity of a heterochiral dodecadeoxynucleotide toward EcoRI that contains an unnatural L-nucleotide residue at the 3'-flanking site of the hexameric EcoRI recognition sequence. In this study, the kinetic parameters for the reaction were determined by means of a heterochiral molecular beacon that contains the EcoRI recognition sequence in the stem region and possesses the fluorescent and quenching dyes at the 5'- and 3'-termini, respectively. We found that the heterochiral molecular beacon showed large V(max) and small K(m) values compared to the corresponding homochiral one. This chiral modification is expected to induce a preferable conformational change for binding and catalysis by EcoRI. (C) 2009 Elsevier Inc. All rights reserved.
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