期刊
CARBOHYDRATE POLYMERS
卷 45, 期 3, 页码 269-275出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0144-8617(00)00259-9
关键词
enzymatic treatment; ferulic acid; maize bran
Different commercial enzyme preparations have been tested to release ferulic acid from maize bran. Best results were obtained with a preparation from Humicola insolens: Novozym 342. However, only 30% of the ferulic acid contained in the bran was released by Novozym 342; moreover, a part (1/3) of the solubilised ferulic acid was still esterified to sugars. Thermal pretreatment (flash-explosion or autoclaving at 160 degreesC) has: been tested in order to improve ferulic acid solubilisation. About 80% of ferulic acid contained in the bran was solubilised by a sequential procedure using first a Rash-explosion pretreatment of the bran (10% w/v in water) at 180 degreesC (1 min) or 190 degreesC (1 min) followed after cooling by Novozym 342 treatment (3% w/w protein/bran at 40 degreesC for 24 h). Flash-explosion treatment solubilised feruloylated substrates and after Novozym 342 action about 1/3 of solubilised ferulic acid was still esterified to sugars. A similar 80% extent of solubilisation of ferulic acid as feruloylated oligosaccharides was also obtained by autoclaving maize bran (10% w/v in water) at 160 degreesC for 1 h. Subsequent treatment with feruloyl esterases was then necessary to get ferulic acid in its free form. (C) 2001 Elsevier Science Ltd. All rights reserved.
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