期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 376, 期 3, 页码 578-583出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.09.030
关键词
Chickpea; Drought stress; Drought tolerance; cDNA library; Expressed sequence tags; Quantitative real-time PCR
资金
- National Science and Technology Ministry [2007BAC15B06, 2006BAD09A04, 2006BAD09A08]
- National Natural Science Foundation of China [30860152]
- Program of Education Department of Xinjiang Autonomous Region [XJEDU2004117]
Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions. To identify the water-stress-induced genes, two non-normalized cDNA libraries were constructed from the seedling leaves of a drought-tolerant chickpea cultivar under PEG-treated and -nontreated conditions. About 2500 clones from each library were selected randomly for sequencing analysis. Based on IDEG6 online software analysis, 92 genes were differentially expressed, and these genes were involved in diverse biological progresses, Such as Metabolism, transcription, signal transduction, protein Synthesis and others. Most of the up-regulated genes were related to drought tolerance, and the down-regulated genes were mainly involved in photosynthesis. The differential expression patterns of five functional unigenes were confirmed by quantitative real-time PCR (qPCR). The results Will help in understanding the molecular basis Of drought tolerance in chickpea. (C) 2008 Elsevier Inc. All rights reserved.
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