期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 21, 期 14, 页码 4470-4481出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.21.14.4470-4481.2001
关键词
-
资金
- NIDDK NIH HHS [R01 DK039773, R56 DK054741, DK 54741, R01 DK054741, DK 39773, DK02356, DK 38452, P01 DK038452, R37 DK039773] Funding Source: Medline
- NINDS NIH HHS [NS 10828, P50 NS010828, F32 NS010828, P01 NS010828] Funding Source: Medline
The group IV cytosolic phospholipase A(2) (cPLA(2)) has been localized to the nucleus (M. R. Sierra-Honigmann, J. R. Bradley, and J. S. Pober, Lab. Investig. 74:684-695, 1996) and is known to translocate from the cytosolic compartment to the nuclear membrane CS. Clover, M. S. de Carvalho, T. Bayburt, M. Jonas, E. Chi, C. C. Leslie, and ill. H. Gelb, J. Biol. Chem. 270:15359-15367, 1995; A. R. Schievella, M. K. Regier, W L. Smith, and L. L. Lin, J. Biol. Chem. 270:30749-30754, 1995). We hypothesized that nuclear proteins interact with cPLA(2) and participate in the functional effects of this translocation. We have identified a nuclear protein, cPLA(2)-interacting protein (PLIP), a splice variant of human Tip60, which interacts with the amino terminal region of cPLA(2). Like Tip60, FLIP cDNA includes the MYST domain containing a C2HC zinc finger and well-conserved similarities to acetyltransferases, Both PLIP and Tip60 coimmunoprecipitate and colocalize with cPLA(2) within the nuclei of transfected COS cells. A polyclonal antibody raised to PLIP recognizes both FLIP and Tip60. Endogenous Tip60 and/or FLIP in rat mesangial cells is localized to the nucleus in response to serum deprivation. Nuclear localization coincides temporally with apoptosis. FLIP expression, mediated by adenoviral gene transfer, potentiates serum deprivation-induced prostaglandin E-2 (PGE(2)) production and apoptosis in mouse mesangial cells from cPLA(2)(+/+) mice but not in mesangial cells derived from cPLA(2)(-/-) mice. Thus FLIP, a splice variant of Tip60, interacts with cPLA(2) and potentiates cPLA(2)-mediated PGE(2) production and apoptosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据