期刊
MICROBIOLOGY-SGM
卷 147, 期 -, 页码 1755-1763出版社
MICROBIOLOGY SOC
DOI: 10.1099/00221287-147-7-1755
关键词
white-rot fungus; transcriptional regulation; ligninolytic enzymes; polyphenol oxidases
类别
Four laccase isozyme genes, Psc lac1,2,3 and 4 have been cloned from the edible mushroom, Pleurotus sajor-caju. The genes display a high degree of homology with other basidiomycete laccases (55-99%) at the amino acid level. Of the laccase genes isolated, Psc lad and 4 displayed the highest degree of National Food similarity (85% at the amino acid level), while Psc lac3 showed the highest degree of divergence, exhibiting only 52-57% amino acid similarity to the other PI. sajor-caju laccase gene sequences. Laccase activity in PI. sajor-caju is affected by nutrient nitrogen and carbon, and by the addition of copper and manganese to the growth medium. In addition, 2,5-xylidine, ferulic acid, veratric acid and 1-hydroxybenzotriazole induced laccase activity in the fungus. Induction of individual laccase isozyme genes by carbon, nitrogen, copper, manganese and the two aromatic compounds, 2,5-xylidine and ferulic acid, occurred at the level of gene transcription. While Psc lac3 transcript levels appeared to be constitutively expressed, transcript levels for the other laccase isozyme genes, lac1, 2 and 4 were differentially regulated under the conditions tested.
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