期刊
CELLULAR SIGNALLING
卷 13, 期 7, 页码 507-513出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0898-6568(01)00167-X
关键词
phosphoric diester hydrolases; two-hybrid; WD-repeat; transducin; tup1; Gs beta
类别
资金
- NIGMS NIH HHS [R01-GM58553] Funding Source: Medline
A strategy of mutagenesis followed by yeast two-hybrid assay was used to determine the sites on the WD-repeat protein Receptor for Activated C Kinase 1 (RACK1) necessary for it to interact with the cAMP-specific phosphodiesterase isoform PDE4D5. Analysis of deletion mutations demonstrated that WD-repeats 5-7, inclusively, of RACK1 contained the major site for interaction with PDE4D5. A reverse two-hybrid screen focusing on WD-repeats 5-7 of RACK1 isolated 11 single amino acid mutations from within this region that blocked the interaction. The ability of these mutations to block the interaction was confirmed by pull-down assays using bacterially expressed glutathione-S-transferase (GST)-RACK1 and mammalian cell-expressed PDE4D5. A model of RACK1 structure. based on the structural similarity of RACK1 to other beta -propeller WD-repeat proteins. indicated that the majority of the amino acids identified by mutagenesis are clustered in a discrete surface of RACK1. We propose that this surface of RACK1 is the major site for its interaction with the unique aminoterminal region of PDE4D5. (C) 2001 Elsevier Science Inc. All rights reserved.
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