期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 374, 期 2, 页码 315-319出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.07.015
关键词
Schizosaccharomyces pombe; plasmid; gene expression; homologous recombination; knock-in
资金
- CREST Research Project
- Japan Science and Technology Agency
- Ministry of Education, Culture, Sports, Science and Technology of Japan
A series of fission yeast targeting vectors that can be used for wild-type strains having no selectable markets have been designed. The functions of one of three market genes, lys1(+), arg1(+), and his3(+), involved in amino acid synthesis, are impaired by integration of the fragments generated by restriction enzyme digestion of the plasmids. Successful integration of the fragments into the targeted loci can be readily verified by their requirement for amino acids, or by the PCR diagnostic analysis. Since these selection markers are not used commonly in fission yeast, these plasmids are likely to facilitate studies that require the co-expression of genes Such as co-localization and CO-immunoprecipitation experiments, by employing them in combination with most of the previously reported markers. (C) 2008 Elsevier Inc. All rights reserved.
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