期刊
PHARMACEUTICAL RESEARCH
卷 18, 期 7, 页码 928-936出版社
KLUWER ACADEMIC/PLENUM PUBL
DOI: 10.1023/A:1010975908915
关键词
dendrimers; FCS; multimolecular complexes; oligonucleotides; cationic polymers
Purpose, To evaluate whether fluorescence correlation spectroscopy (FCS) can be used to characterize the complexation between oligonucleotides and cationic polymers. Methods. The features of the complexes between rhodamine labeled oligonucleotides (Rh-ONs) and poly(2-dimethylamino)ethyl methacrylate (pDMAEMA), poly(ethylene glycol)-poly(ethyleneimine) (pEG-pEI), and diaminobutane-dendrimer-(NH2)(64) (DAB(64)) were characterized by light scattering, electrophoretic mobility, elcctrophoresis, and FCS. Results. At low polymer/Rh-ON ratios, a decrease of the fluorescence of the Rh-ONs was observed on binding of the Rh-ONs to all cationic polymers. This was explained by the creation of multimolecular complexes in which the Rh-labels quench each other. The multimolecular complexes, which are highly fluorescent as they carry a number of Rh-ONs, resulted in high fluorescence peaks in the fluorescence fluctuation profile as measured by FCS. For pDMAEMA and DAB(64), at higher polymer/Rh-ON ratios the fluorescence of the polyplexes increased, caused by the formation of mono-molecular complexes. which consist of only one Ph-ON per poly mer. In the case of pEG-pEI, the fluorescence stayed constant when the polymer/Rh-ON ratio increased, so multimolecular polyplexes remained. FCS confirmed these results as the high fluorescence peaks disappeared in case of pDMAEMA/Rh-ON and DAB(64)/Rh-ON dispersions, but remained present for pEG-pEI/Rh-ON dispersions. Conclusions. FCS seems applicable for study of the interactions between ONs and different types of cationic polymers.
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