期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 372, 期 4, 页码 589-594出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.05.094
关键词
packaging RNA; packaging motor; DNA packaging; bacteriophage; phi29; RNA assembly; non-coding RNA
资金
- NEI NIH HHS [PN2 EY018230, PN2 EY 018230] Funding Source: Medline
- NIBIB NIH HHS [R01 EB003730] Funding Source: Medline
- NIGMS NIH HHS [R01 GM069557, GM069557, GM059944, R01 GM069557-02, R01 GM059944] Funding Source: Medline
The bacteriophage phi29 DNA packaging motor is a protein/RNA complex that can produce strong force to condense the linear-double-stranded DNA genome into a pre-formed protein capsid. The RNA component, called the packaging RNA (pRNA), utilizes magnesium-dependent inter-molecular base-pairing interactions to form ring-shaped complexes. The pRNA is a class of non-coding RNA, interacting with phi29 motor proteins to enable DNA packaging. Here, we report a two-piece chimeric pRNA construct that is fully competent in interacting with partner pRNA to form ring-shaped complexes, in packaging DNA via the motor, and in assembling infectious phi29 virions in vitro. This is the first example of a fully functional pRNA assembled using two non-covalently interacting fragments. The results support the notion of modular pRNA architecture in the phi29 packaging motor. (C) 2008 Elsevier Inc. All rights reserved.
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