期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 14, 页码 7492-7496出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b00199
关键词
graphene oxide; bisphenol A; fluorescence; biosensor; FAM-ssDNA
资金
- MOST [2012AA06A303]
- National Natural Science Foundation of China [31401566]
- NSF of Jiangsu Province [BK20130379, 13KJB550001, BK20140416]
- Suzhou Science and Technology Committee Program [SS201335, SYN201210]
Bisphenol A (BPA) is an important industrial chemical for polycarbonate (PC) and epoxy resins in paper and plastic industries. In our work, a kind of new method for detection of BPA was designed based on graphene oxide and anti-BPA aptamer. The graphene oxide can specifically adsorb and quench the fluorescence of fluorescently modified ssDNA probes. Meanwhile, the BPA can combine with anti-BPA optamer and switch its configuration to prevent the aptamer from adsorbing on the surface of graphene oxide (GO). Under different concentrations of BPA, based on the target-induced conformational change of anti-BPA aptamer and the interactions between the fluorescently modified anti-BPA aptamer (FAM-ssDNA) and GO, the experimental results show that the intensity of the fluorescence signal was changed. A low limit of detection of 0.05 ng/mL was obtained in the range 0.110 ng/mL. In addition, the specificity was outstanding among analogues of BPA. The recovery rate in actual water samples spiked with BPA can be 96.0% to 104.5%. The developed method was successfully used to determine BPA in actual water samples.
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