期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 377, 期 2, 页码 573-578出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.10.038
关键词
Rap2; Rap1; Ras; Small G protein; MINK; TNIK; Mitogen-activated protein kinase kinase kinase kinase; TANC1; Postsynaptic scaffold protein
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18013044]
- Japan Society for the Promotion of Science (JSPS). [19790794, 18590303, 20590311]
- Grants-in-Aid for Scientific Research [20590311, 19790794, 18013044, 18590303] Funding Source: KAKEN
Rap1 and Rap2 are similar Ras-like G proteins but perform distinct functions. By the affinity chromatography/mass-spectrometry approach and the yeast two-hybrid screening, we identified Misshapen/NIKs-related kinase (MINK) as a novel Rap2-interacting protein that does not interact with Rap1 or Ras. MINK is a member of the STE20 group of mitogen-activated protein kinase kinase kinase kinases. The interaction between MINK and Rap2 was GTP-dependent and required Phe39 within the effector region of Rap2; the corresponding residue in Rap1 and Ras is Set. MINK was enriched in the brain, and both MINK and its close relative, Traf2- and Nck-interacting kinase (TNIK), interacted with a postsynaptic scaffold protein containing tetratricopeptide repeats, ankyrin repeats and a coiled-coil region (TANC1) and induced its phosphorylation, under control of Rap2 in cultured cells. These are novel actions of MINK and TNIK, and consistent with a role of MINK as a Rap2 effector in the brain. (C) 2008 Eisevier Inc. All rights reserved.
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