期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 58, 期 8, 页码 1150-1158出版社
BIRKHAUSER VERLAG AG
DOI: 10.1007/PL00000929
关键词
hypoxia; stem cell; cellular senescence; aging; cell cycle; Akt
资金
- NIA NIH HHS [AG18780] Funding Source: Medline
The proliferation ability of satellite cells (considered the 'stem cells' of mature myofibers) declines with increasing age when cultured under standard cell culture conditions of 21 % oxygen. However, actual oxygen levels in the intact myofiber in vivo are an order of magnitude lower. No studies to date have addressed the issue of whether culturing satellite cells from old muscles under more 'physiologic' conditions would enhance their proliferation and/or differentiation ability. Therefore, we analyzed satellite cells derived from 31-month-old rats in standard cultures with 21 % O-2 and in lowered (similar to3 %) O-2. Under the lowered O-2 conditions, we noted a remarkable increase in the percentage of large-sized colonies, activation of cell cycle progression factors, phosphorylation of Akt, and downregulation of the cell cycle inhibitor p27(Kip1). These data suggest that lower O-2 levels provide a milieu that stimulates proliferation by allowing continued cell cycle progression, to result ultimately in the enhanced in vitro replicative life span of the old satellite cells. Such a method therefore provides an improved means for the ex vivo generation of progenitor satellite cell populations for potential therapeutic stem cell transplantation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据