期刊
EMBO JOURNAL
卷 20, 期 13, 页码 3587-3595出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/20.13.3587
关键词
glucose transporter; glycolysis; mRNA degradation; PTS; RNase E
We report a novel post-transcriptional control of the ptsG gene encoding the major glucose transporter IICBGlc. We demonstrate that the level of IICBGlc is markedly reduced when the glycolytic pathway is blocked by a mutation in either the pgi or pfkA gene encoding phosphoglucose isomerase or phosphofructokinase, respectively. This down-regulation of ptsG is not exerted at the transcriptional level. Both northern blot and S1 analyses demonstrate that the mutation dramatically accelerates the degradation of ptsG mRNA, The degradation of ptsG mRNA occurs in wild-type cells when a-methylglucoside, a nonmetabolizable analog of glucose, is present in the medium. The addition of any one of the glycolytic intermediates downstream of the block prevents the degradation of ptsG mRNA, The rapid degradation of ptsG mRNA is eliminated when RNase E is thermally inactivated. We conclude that the glycolytic pathway controls ptsG expression by modulating RNase E-mediated mRNA degradation. This is the first instance in which the glycolytic flux has been shown to affect the expression of a specific gene through mRNA stability.
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