期刊
EMBO JOURNAL
卷 20, 期 13, 页码 3351-3358出版社
WILEY-BLACKWELL
DOI: 10.1093/emboj/20.13.3351
关键词
cultured cell; prion disease; prion protein isoforms; scrapie strain; transmissible spongiform encephalopathy
Bovine spongiform encephalopathy (BSE) and its human equivalent, variant Creutzfeldt-Jakob disease (vCJD), are caused by the same strain of infectious agent, which is similar to, but distinct from, > 20 strains of their sheep scrapie homologue, A better understanding of the molecular strain determinants could be obtained from cells in monoculture than from whole animal studies where different cell targeting is commonly a strain-related feature. Although a few cell types can be infected with different strains, the phenotypes of the emergent strains have not been studied. We have cured the scrapie-infected, clonal SMB cell line with pentosan sulfate, stably re-infected it with a different strain of scrapie and shown that biological properties and prion protein profiles characteristic of each original strain are propagated faithfully in this single non-neuronal cell type. These findings attest to the fact that scrapie strain determinants are stable and host-independent in isolated cells.
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