期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 367, 期 4, 页码 755-760出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2008.01.013
关键词
Bombyx mori; argonaute family; Piwi; AGO3; piRNA; oogenesis; spermatogenesis; ovary; testis
The Piwi subfamily proteins and their partner, Piwi-interacting RNAs (piRNAs), play an important roles in germ-line development and silencing of selfish DNA elements. To date, however, the developmental expression profiles of the Piwi subfamily genes are poorly known. In this study, we examined the expression profiles of two Bombyx mori Piwi subfamily genes, silkworm Piwi (SIWI) and BmAGO3, which are possible partners of Bombyx piRNA-like small RNAs we recently identified in B. mori germ-line cells. Quantitative reverse transcription-polymerase chain reaction analyses demonstrated that these two genes were abundantly expressed in the larval testis, pupal ovary, and adult eggs, suggesting that they might be involved in spermatogenesis and oogenesis in Bombyx. Notably, developmental expression patterns of SIWI and BmAGO3 were remarkably similar. Collectively, our results suggest that SIWI and BmAGO3 may cooperate in an unknown pathway during the development of B. mori germ-line cells. (c) 2008 Elsevier Inc. All rights reserved.
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