期刊
JOURNAL OF CHROMATOGRAPHY A
卷 921, 期 2, 页码 109-119出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)00860-3
关键词
adsorption; kinetic studies; poly(vinyl alcohol) sorbent; affinity sorbents; magnetic sorbents; proteins
A poly(vinyl alcohol)-based magnetic gel entrapping Fe3O4 colloids has been prepared by an emulsification-crosslinking method. The gel was modified with Cibacron blue 3GA, and thus a magnetic affinity support was produced. The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin obeyed the Henry's law. Uptake kinetics of the two proteins was investigated and analyzed with a pore diffusion model and a homogeneous diffusion model. Experimental results showed that the magnetic affinity gel had magnetic responsiveness and favorable properties in protein adsorption, and was mechanically and chemically stable. (C) 2001 Elsevier Science B.V. All rights reserved.
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