期刊
BIOCATALYSIS AND BIOTRANSFORMATION
卷 28, 期 4, 页码 227-234出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/10242422.2010.493208
关键词
gamma-Decalactone; lipase; methyl ricinoleate; oxygen; Yarrowia lipolytica
资金
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BD/28039/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/28039/2006] Funding Source: FCT
Yarrowia lipolytica was used to produce gamma-decalactone by the degradation of methyl ricinoleate (MR). A new method for inoculating the biotransformation medium was tested, which avoided the laborious step of washing cells from the growth medium. The consequent cell hydrophobicity increase led to an enhancement of aroma production. In a study of MR concentration in shake flasks, the highest productivity (15 mg L-1 h(-1)) was achieved using 30 g MR L-1. Lipase and protease activities were induced but no correlation between lipase induction and aroma production was found. The effects of different aeration and agitation rates were studied in bioreactor assays. Productivity was improved to 87 mg L-1 h(-1), and another compound, 3-hydroxy-gamma-decalactone, was detected in large amounts. Dehydration of this lactone produced two decenolides with aroma characteristics. The direct influence of oxygen on the production of both lactones was demonstrated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据