期刊
BIOCATALYSIS AND BIOTRANSFORMATION
卷 26, 期 1-2, 页码 133-140出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420701789411
关键词
glycogen; branching enzyme; alpha-glucan; hyperbranched polymer; production process
Two well-established methods to prepare glycogen are available: (1) extraction from natural resources such as shellfish and animal tissues; (2) synthesis from glucose-1-phosphate using two enzymes, alpha-glucan phosphorylase (EC 2.4.1.1) and branching enzyme (EC 2.4.1.18). We have developed a novel enzymatic process for glycogen production, in which short-chain amylose is first prepared from starch or dextrin by using isoamylase (EC 3.2.1.68), and then branching enzyme and amylomaltase (EC 2.4.1.25) are added to synthesize glycogen. Our enzymatic process, using isoamylase, branching enzyme and amylomaltase, is currently the most efficient for glycogen production. Furthermore, the molecular weight of glycogen is controllable in a range of 3.0 x 10(6) to 3.0 x 10(7) by adjusting some parameters of the reaction.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据