期刊
CELLULAR IMMUNOLOGY
卷 211, 期 1, 页码 37-42出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/cimm.2001.1828
关键词
interleukin-13 (IL-13); IL-13 antagonist; IL-13 cytotoxin; Hodgkin lymphoma; Hodgkin cells; Reed-Sternberg cells; Hodgkin/Reed-Sternberg tumor cells; autocrine; paracrine
IL-13 has been proposed to be an autocrine growth factor for Hodgkin/Reed-Sternberg tumor cells (H/RS cells). Since we have recently identified and produced a novel IL-13 antagonist (IL-13E13K) that can suppress the biological activity of IL-13, here we examined whether IL-13E13K can inhibit growth of Hodgkin lymphoma (HL)-derived cell lines. IL-13E13K not only inhibited the growth of an unstimulated H/RS cell line (L1236) but also cells that were stimulated by exogenous IL-13 in a dose-dependent manner. Several HL-derived cell lines expressed IL-13 message and protein and message for various chains of IL-13R. FURS cell lines expressed mRNA for the IL-13R alpha1, IL-4R alpha, and IL-2R gamma chains. However, none of these cell lines expressed the IL-13R alpha2 chain. An H/R,S cell line (L1236) internalized the ligand-receptor complex after binding to a fusion protein composed of IL-13 and a mutated form of Pseudomonas exotoxin A (IL-13PE38QQR, or IL-13 cytotoxin), as IL-13 cytotoxin was specifically cytotoxic to H/RS cells an vitro. These results indicate that IL-13E13K and IL-13 cytotoxin can effectively suppress growth of a L1236 MRS cell line. Therefore, additional studies should be performed to determine the expression of IL-13 and IL-13R in primary clinical samples of Hodgkin's lymphoma and both agents should be further tested in vitro and an vivo as possible therapeutic agents for HL.
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