期刊
GENE
卷 272, 期 1-2, 页码 227-235出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-1119(01)00535-2
关键词
kil; gam; cell division; flow cytometry
Induction of a lambda prophage causes the death of the host cell even in the absence of phage replication and lytic functions due to expression of functions from the lambdap(L) operon. We genetically modified the lambda prophage to determine which lambdap(L) operon functions were involved in cell killing. Viability assays and How cytometry were used to monitor cell death and filamentation. The kil gene was shown to cause cell death and filamentation as described previously. Another killing activity was mapped within the PL operon to the gam gene. Inspection of the DNA sequence showed that there are two possible translation start points for both kil and gain. In both cases, the shorter of the two possible products could cause cell killing. The shorter products were also sufficient for the known filamentation and recombination activities of the respective Kil and Gam functions. The expression level of the p(L) operon is down-regulated by Cro repressor. In the absence of Cro, higher P-L expression levels allow either Kil or Gam to be lethal or growth inhibitory, whereas at lowered expression in Cro-repressed conditions, only Kil is lethal. The filamentation function of Kil and recombination activity of Gam are unaffected at Cro-repressed levels of expression. Published by Elsevier Science B.V.
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