期刊
BRAIN RESEARCH
卷 907, 期 1-2, 页码 93-99出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(01)02607-5
关键词
capillary; ischemia; potassium channel; retina; vascular; adenosine receptor
资金
- NEI NIH HHS [EY12505, EY07003] Funding Source: Medline
- NIDDK NIH HHS [DK-20572] Funding Source: Medline
In the CNS, contractile pericytes are positioned on the endothelial walls of microvessels where they are thought to play a role in adjusting blood flow to meet local metabolic needs. This function may be particularly important in the retina where pericytes are more numerous than at any other site, Despite the putative importance of pericytes, knowledge of the mechanisms by which vasoactive molecules, such as adenosine, regulate their function is limited. Using the perforated-patch configuration of the patch-clamp technique to monitor the whole-cell currents of pericytes located on microvessels freshly isolated from the adult rat retina, we found that adenosine reversibly activated a hyperpolarizing current in 98% of the sampled pericytes. This adenosine-induced current is likely to be due to the opening of ATP-sensitive potassium (K-ATP) channels since it had a reversal potential near the equilibrium potential for K+, was inhibited by the K-ATP channel blocker, glibenclamide, and was mimicked by pinacidil, which is a K-ATP channel opener. Experiments with specific agonists and antagonists indicated that both the high affinity A(1) and the lower affinity A(2a) adenosine receptors provided effective pathways for activating K-ATP currents in pericytes recorded under normal metabolic conditions. However, during chemical ischemia, the A(1) receptor pathway rapidly became ineffective. In contrast, activation of A(2a) adenosine receptors continued to open K-ATP channels in ischemic pericytes. These results suggest that the regulation of K-ATP channels via A(1) and A(2a) receptors allows adenosine to serve over a broad range of metabolic conditions as a vasoactive signal in the retinal microvasculature. (C) 2001 Published by Elsevier Science B.V.
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