期刊
ANALYTICAL CHEMISTRY
卷 73, 期 14, 页码 3294-3299出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac010143j
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资金
- NIGMS NIH HHS [5 R44 GM56580-03, 2 R44 GM56603-02, R01 GM55872-02] Funding Source: Medline
A high-throughput mass spectrometric immunoassay (MSIA) system for the analysis of proteins directly from biological fluids is reported. A 96-well-format robotic workstation equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of specific proteins from samples and subsequent deposition onto 96-well arrayed matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) targets. Interferences:from nonspecificalIy bound proteins were minimized through choice of appropriate affinity pipet tip derivatization chemistries, Sample preparation for MALDI-TOFMS was enhanced through the use of hydrophobic/hydrophilic contrasting targets, which also presented functionalities found to promote matrix/analyte crystal growth, Automated mass spectrometry was used in the unattended acquisition of data, resulting in an analysis rate of similar to 100 samples/h (biological fluid --> data). The quantitative MSIA of beta (2)m levels present in human plasma samples is given as illustration.
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