期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 391, 期 2, 页码 180-187出版社
ACADEMIC PRESS INC
DOI: 10.1006/abbi.2001.2415
关键词
cytochrome P450; CYP94A2; Vicia sativa; fatty acid hydroxylase; omega-hydroxylation; (omega-1)-hydroxylation; site-directed mutagenesis; SRS-6; Phe494; regioselectivity; active site
Fatty acid omega -hydroxylation is involved in the biosynthesis of the plant cuticle, formation of plant defense signaling molecules, and possibly in the rapid catabolism of free fatty acids liberated under stress conditions. CYP94A2 is a cytochrome P450-dependent medium-chain fatty acid hydroxylase that was recently isolated from Vicia sativa, Contrary to CYP94A1 and CYP86A1, two other fatty acid hydroxylases previously characterized in V. sativa and Arabidopsis thaliana, CYP94A2 is not a strict omega -hydroxylase, but exhibits chain-length-dependent regioselectivity of oxidative attack. Sequence alignments of CYP94A2 with CYP94A1 and molecular modeling studies suggested that F494, located in SRS-B (substrate recognition site) was involved in substrate recognition and positioning. Indeed, a conservative amino acid substitution at that position markedly altered the regiospecificity of CYP94A2. The observed shift from omega toward omega -1 hydroxylation was prominent with lauric acid as substrate and declined with increasing fatty acid chain length. (C) 2001 Academic Press.
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