期刊
EMBO JOURNAL
卷 20, 期 14, 页码 3840-3848出版社
WILEY
DOI: 10.1093/emboj/20.14.3840
关键词
HCV core protein; HCVF protein; hepatitis C virus; ribosomal frameshift
资金
- NIAID NIH HHS [N01AI40038, U019AI40038, R03AI45873] Funding Source: Medline
Hepatitis C virus (HCV) is an important human pathogen that affects similar to 100 million people worldwide. Its RNA genome codes for a polyprotein, which is cleaved by viral and cellular proteases to produce at least 10 mature viral protein products. We report here the discovery of a novel HCV protein synthesized by ribosomal frameshift. This protein, which we named the F protein, is synthesized from the initiation codon of the polyprotein sequence followed by ribosomal frameshift into the -2/+1 reading frame. This ribosomal frameshift requires only codons 8-14 of the core protein-coding sequence, and the shift junction is located at or near codon 11. An F protein analog synthesized in vitro reacted with the sera of HCV patients but not with the sera of hepatitis B patients, indicating the expression of the F protein during natural HCV infection. This unexpected finding may open new avenues for the development of anti-HCV drugs.
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