期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 67, 期 1-2, 页码 97-106出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1605(01)00432-9
关键词
Bifidobacterium longum SBT2928 (strain Yukijirushi); enterotoxigenic Escherichia coli; gangliotetraosylceramide; binding-inhibitory factor (BIF)
The extracellular protein fraction (P100V) containing the protein BIF produced by Bifidobacterium longum SBT2928 (BL2928), which inhibits the binding of enterotoxigenic Escherichia coli Pb176 (ETEC) to the glycolipid binding receptor gangliotetraosylceramide (GA1) also inhibited the binding of ETEC to the human intestinal epithelial cell line HCT-8 (ATCC CCL 244) in a dose-dependent manner. ETEC-binding inhibitory experiments using crude colonization factor antigen (CFA)-II prepared from ETEC, rabbit anti-GA1 antiserum, medium containing GA1 and media containing lectins, as the binding-inhibitors, suggest that the interaction between the CFA-II antigen present on the cell surface of ETEC and GA1 expressed on HCT-8 cells plays a significant role in the adherence between them. It is strongly suggested that the P100V fraction works as a blocker for the ETEC receptor GA1 on HCT-8 cells. (C) 2001 Elsevier Science B.V. All rights reserved.
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