期刊
CELL
卷 106, 期 2, 页码 157-169出版社
CELL PRESS
DOI: 10.1016/S0092-8674(01)00421-4
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资金
- NIAID NIH HHS [AI34867] Funding Source: Medline
Plasma membrane wounds are repaired by a mechanism involving Ca2+-regulated exocytosis. Elevation in intracellular [Ca2+] triggers fusion of lysosomes with the plasma membrane, a process regulated by the lysosomal synaptotagmin isoform Syt VII. Here, we show that Ca2+-regulated exocytosis of lysosomes is required for the repair of plasma membrane disruptions. Lysosomal exocytosis and membrane resealing are inhibited by the recombinant Syt VII C(2)A domain or anti-Syt VII C(2)A antibodies, or by antibodies against the cytosolic domain of Lamp-1, which specifically aggregate lysosomes. We further demonstrate that lysosomal exocytosis mediates the resealing of primary skin fibroblasts wounded during the contraction of collagen matrices. These findings reveal a fundamental, novel role for lysosomes: as Ca2+-regulated exocytic compartments responsible for plasma membrane repair.
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