期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 98, 期 16, 页码 9348-9352出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.151247598
关键词
-
资金
- NIGMS NIH HHS [R37 GM027099, R01 GM027099, GM27099] Funding Source: Medline
Holins comprise the most diverse functional group of proteins known. They are small bacteriophage-encoded proteins that accumulate during the period of fate-protein synthesis after infection and cause lysis of the host cell at a precise genetically programmed time, ft is unknown how holins achieve temporal precision, but a conserved feature of their function is that energy poisons subvert the normal scheduling mechanism and instantly trigger membrane disruption. On this basis, timing has been proposed to involve a progressive decrease in the energized state of the membrane until a critical triggering level is reached. Here, we report that membrane integrity is not compromised after the induction of holin synthesis until seconds before lysis. The proton motive force was monitored by the rotation of individual cells tethered by a single flagellum. The results suggest an alternative explanation for the lysis clock, in which holin concentrations build to a critical level that leads to formation of an oligomeric complex that disrupts the membrane.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据