期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
卷 130, 期 1, 页码 81-91出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S1095-6433(01)00367-1
关键词
sodium/proton exchange; NHE; agnatha; hagfish; gills; acid-base regulation; pH lance; epithelial transport
Sodium/proton exchangers (NHE) are transmembrane proteins that facilitate the exchange of a Na+ ion for a H+ ion across cellular membranes. ne NHE are present in the gills of fishes and are believed to function in acid-base regulation by driving the extrusion of protons across the branchial epithelium in exchange for Na+ in the water. In this study, we have used reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the presence of a branchial NHE in the gills of the Atlantic hagfish, Myxine glutinosa. The subsequent partial cDNA sequence shares homology with other vertebrate and invertebrate NHE isoforms. In addition, using semi-quantitative, multiplex RT-PCR we demonstrate that mRNA expression of hagfish gill NHE is upregulated following an induced metabolic acidosis. Expression was increased to 4.4 times basal levels at 2-h post-infusion and had decreased to 1.6 times basal by 6 h. Expression had returned to basal levels by 24-h post-infusion. The inference from this study is that a gill NHE which is potentially important in acid-base regulation has been present in the vertebrate lineage since before the divergence of the hagfishes from the main vertebrate line. (C) 2001 Elsevier Science Inc. All rights reserved.
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