期刊
FEMS MICROBIOLOGY ECOLOGY
卷 37, 期 1, 页码 21-29出版社
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6941.2001.tb00849.x
关键词
rhizosphere; biodiversity; amplified ribosomal DNA restriction analysis; repetitive extragenic patindromic-PCR; Arabidopsis thaliana; Pseudomonas brassicacearum
类别
Pseudomonas brassicacearum is a newly described bacterial species isolated from the rhizosphere of Arabidopsis thaliana. The P. brassicacearum populations were isolated from the rhizosphere of two ecotypes of A. thaliana (Wassilewskija (WS) and Columbia (COL)), a mutant of Columbia impaired in starch metabolism (pgm mutant), and a genetically distant plant (wheat), grown in a French eutric cambisol (Mereville). The strains were isolated on semi-selective media. Their diversity was assessed using repetitive extragenic palindromic (REP)-PCR profiling and their affiliation to the P. brassicacearum species using ARDRA and siderotyping. A total of 379 strains isolated in two experiments were clustered into 68 REP-genotypes. Statistical analysis showed that the genetic structure of the P. brassicacearum populations was homogeneous for strains isolated from different plants of the same genotype within the same experiment, but significantly differed across the four tested plant genotypes. Comparison of the REP-genotype distributions showed that some bacterial genotypes were poorly represented, whereas others were strongly stimulated by plant roots. (C) 2001 Federation of European Micro biological Societies. Published by Elsevier Science B.V. All rights reserved.
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