期刊
ANALYTICAL BIOCHEMISTRY
卷 295, 期 1, 页码 88-100出版社
ACADEMIC PRESS INC
DOI: 10.1006/abio.2001.5178
关键词
electrospray ionization; mass spectrometry; tandem mass spectrometry; triacylglyceride molecular species; rat myocardium
资金
- NHLBI NIH HHS [R02 HL 41250, P01 HL57278/] Funding Source: Medline
- NIAAA NIH HHS [R01 AA11094] Funding Source: Medline
- PHS HHS [JDFI 996003] Funding Source: Medline
Herein we describe a rapid, simple, and reliable method for the quantitative analysis and molecular species fingerprinting of triacylglycerides (TAG) directly from chloroform extracts of biological samples. Previous attempts at direct TAG quantitation by positive-ion electrospray ionization mass spectrometry (ESI/MS) were confounded by the presence of overlapping peaks from choline glycerophospholipids requiring chromatographic separation of lipid extracts prior to ESI/MS analyses. By exploiting the rapid loss of phosphocholine from choline glycerophospholipids, in conjunction with neutral-loss scanning for individual fatty acids, overlapping peaks in the ESI mass spectrum were deconvoluted generating a detailed molecular species fingerprint of individual TAG molecular species directly from chloroform extracts of biological samples. This method readily detects as little as 0.1 pmol of each TAG molecular species from chloroform extracts and is linear over a 1000-fold dynamic range. The sensitivity of individual TAG molecular species to ESI/MS/MS analyses correlated with the unsaturation index and inversely correlated with total aliphatic chain length of TAG. An algorithm was developed which identifies sensitivity factors, thereby allowing the rapid quantitation and molecular species fingerprinting of TAG molecular species directly from chloroform extracts of biological samples. (C) 2001 Academic Press.
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