期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 534, 期 3, 页码 641-650出版社
WILEY
DOI: 10.1111/j.1469-7793.2001.t01-1-00641.x
关键词
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1. We characterized the mechanisms in vascular smooth muscle cells (VSMCs) that produce asynchronous, wave-like Ca2+ oscillations in response to phenylephrine (PE). Confocal imaging was used to observe [Ca2+](i) in individual VSMCs of intact inferior vena cava (IVC) from rabbits. 2. It was found that the Ca2+ waves were initiated lay Ca2+ release from the sarcoplasmic reticulum (SR) via inositol 1,4,5-trisphosphate-sensitive SR Ca2+ release channels (IP3R channels) and that refilling of the SR Ca2+ store through the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA) was required for maintained generation of the repetitive Ca2+ waves. 3. Blockade of L-type voltage-gated Ca2+ channels (L-type VGCCs) with nifedipine reduced the frequency of PE-stimulated [Ca2+](i) oscillations, while additional blockade of receptor-operated channels/store-operated channels (ROCs/SOCs) with SKF96365 abolished the remaining oscillations. Parallel force measurements showed that nifedipine inhibited PE-induced tonic contraction by 27% while SKF96365 abolished it. This indicates that stimulated Ca2+ entry refills the SR to support the recurrent waves of SR Ca2+ release and that both L-tyke VGCCs and ROCs/SOCs contribute to this process: 4. Application of the Na+-Ca2+ exchanger (NCX) inhibitors 2',4'-dichlorobenzamil (forward- and reverse-mode inhibitor) and KB-R7943 (reverse-Triode inhibitor) completely abolished the nifedipine-resistant, component of [Ca2+](i) oscillations and markedly reduced PE-induced tone. 5. Thus, we conclude that, each Ca2+ wave depends on initial SR Ca2+ release via IP3R channels followed by SR Ca2+ refilling through SERCA. Na+ entry through ROCs/SOCs facilitates Ca2+ entry through the NCX operating in the reverse anode, which refills the SR and maintains PE-induced [Ca2+](i) oscillations. In addition some Ca2+ entry through L-type VGCCs and ROCs/SOCs serves to modulate the frequency of the oscillations and the magnitude of force development.
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