期刊
BEST PRACTICE & RESEARCH CLINICAL HAEMATOLOGY
卷 24, 期 1, 页码 73-81出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.beha.2010.11.002
关键词
mesenchymal stromal cells; ex vivo expansion; fetal calf serum; platelet lysate; malignant transformation; cellular therapy
类别
资金
- Istituto Superiore di Sanita (National Program on Stem Cells)
- MIUR (Ministero dell'Istruzione, dell'Universita e della Ricerca. Progetti di Rilevante Interesse Nazionale, PRIN)
- Associazione Italiana per la Ricerca sul Cancro (AIRC) [IG6071, IG9062]
Mesenchymal stromal cells (MSCs) are adult multipotent cells that can be isolated from several human tissues. MSCs represent a novel and attractive tool in strategies of cellular therapy. For in vivo use, MSCs have to be ex vivo expanded in order to reach the numbers suitable for their clinical application. Despite being efficacious, the use of fetal calf serum for MSC ex vivo expansion for clinical purposes raises concerns related to immunization and transmission of zoonoses; the standardization of expansion methods, possibly devoid of animal components, such as those based on platelet lysate, are discussed in this paper. Moreover, this review focuses on the search of novel markers for the prospective identification/isolation of MSCs and on the potential risks connected with ex vivo expansion of MSCs, in particular that of their malignant transformation. Available tests to study the genetic stability of ex vivo expanded MSCs are also analyzed. (C) 2010 Elsevier Ltd. All rights reserved.
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