Novel genes of the sox gene cluster, mutagenesis of the flavoprotein SoxF, and evidence for a general sulfur-oxidizing system in Paracoccus pantotrophus GB17

The novel genes soxFGH were identified, completing the sox gene cluster of Paracoccus pantotrophus coding for enzymes involved in lithotrophic sulfur oxidation, The periplasmic SoxF, SoxG, and SoxH proteins were induced by thiosulfate and purified to homogeneity from the soluble fraction, soxF: coded for a protein of 420 amino acids with a signal peptide containing a twin-arginine motif, SoxF was 37% identical to the flavoprotein FccB of flavocytochrome c sulfide dehydrogenase of Allochromatium vinosum. The mature SoxF (42,832 Dal contained 0.74 mot of flavin adenine dinucleotide per moi, soxG coded for a novel protein of 303 amino acids with a signal peptide containing a twin-arginine motif, The mature SoxG (29,657 Dal contained two zinc binding motifs and 0.90 atom of zinc per subunit of the homodimer, soxH coded for a periplasmic protein of 317 amino acids with a double-arginine signal peptide. The mature SoxH (32,317 Dal contained two metal binding motifs and 0.29 atom of zinc and 0.20 atom of copper per subunit of the homodimer, SoxXA, SoxYZ, SoxB, and SoxCD (C, G, Friedrich, A. Quentmeier, F, Bardischewsky, D, Pother, R, Kraft, S, Kostka, and H, Print, J, Bacteriol. 182:4476-4487, 2000) reconstitute a system able to perform thiosulfate-, sulfite-, sulfur-, and hydrogen sulfide-dependent cytochrome c reduction, and this system is the first described for oxidizing different inorganic sulfur compounds. SoxF slightly inhibited the rate of hydrogen sulfide oxidation but not the rate of sulfite or thiosulfate oxidation, From use of a homogenote mutant with an in-frame deletion in soxF and complementation analysis, it was evident that the soxFGH gene products were not required for lithotrophic growth with thiosulfate.