期刊
PLANT AND CELL PHYSIOLOGY
卷 42, 期 8, 页码 879-883出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pce119
关键词
2D-PAGE; disulfide bond reduction; monobromobimane; protein mobilization; rice glutelin; thioredoxin
The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.
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