4.7 Article

Redox changes accompanying the degradation of seed storage proteins in germinating rice

期刊

PLANT AND CELL PHYSIOLOGY
卷 42, 期 8, 页码 879-883

出版社

OXFORD UNIV PRESS
DOI: 10.1093/pcp/pce119

关键词

2D-PAGE; disulfide bond reduction; monobromobimane; protein mobilization; rice glutelin; thioredoxin

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The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.

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