期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 759, 期 1, 页码 179-183出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00204-3
关键词
2-hydroxyflutamide
dFlutamide is a potent antiandrogen used for the treatment of prostatic cancer. Flutamide undergoes extensive first-pass metabolism to the pharmacologically active metabolite 2-hydroxyflutamide. A simple, sensitive, precise, accurate and specific HPLC method, using carbamazepine as the internal standard, for the determination of 2-hydroxyflutamide in human plasma was developed and validated. After addition of the internal standard, the analytes were isolated from human plasma by liquid-liquid extraction. The method was linear in the 25 to 1000 ng/ml concentration range (r>0.999). Recovery for 2-hydroxyflutamide was greater than 91.4% and for internal standard was 93.6%. The limit of quantitation was 25 ng/ml. Inter-batch precision, expressed as the relative standard deviation (RSD), ranged from 4.3 to 7.9%, and accuracy was better than 93.9%. Analysis of 2-hydroxyflutamide concentrations in plasma samples from 16 healthy volunteers following oral administration of 250 mg of flutamide provided the following pharmacokinetic data (mean +/- SD): C-max, 776 +/- 400 ng/ml; AUC(0-infinity), 5368 +/- 2689 ng h/ml; AUC(0-t), 5005 +/- 2605 ng h/ml; T-max, 2.6 +/-1.6 h; elimination half-life, 5.2 +/-2.0 h. (C) 2001 Elsevier Science B.V. All rights reserved.
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