期刊
JOURNAL OF CHROMATOGRAPHY A
卷 926, 期 1, 页码 29-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(01)00806-8
关键词
mass spectrometry; collision-induced dissociation; flavonoids; glycosides
LC-MS-MS is becoming a very important tool for the on-line identification of natural products in crude plant extracts. For an efficient use of this technique in the dereplication of natural products, a careful study of the parameters used to generate informative MS-MS spectra is needed. In this paper, the collision-induced dissociation (CED) MS-MS spectra of ubiquitous C-glycosidic flavonoids have been systematically studied using hybrid quadrupole time-of-flight and ion-trap (IT) mass analysers under various CID energy conditions. Efficient differentiation of flavonoid C-glycoside isomers was possible, based on the comparison of CID-MS-MS spectra of particular C-glycoside unit fragments. Striking differences between 6-C and 8-C flavonoid glycosides were especially observed in the product ion spectra of their X-0,2(+) fragments ([M+H-120](+)). Some guidelines for the on-line characterisation of C-glycosidic flavonoids by LC-MS-MS or LC-multiple-stage MS are given. (C) 2001 Elsevier Science B.V. All rights reserved.
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