期刊
FEBS LETTERS
卷 503, 期 1, 页码 80-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(01)02701-6
关键词
biochemical engineering; N-propanoylmannosamine; sialic acid; UDP-N-acetylglucosamine 2-epimerase
Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. N-Propanoylmannosamine, an unphysiological sialic acid precursor, is taken up and metabolized by mammalian cells resulting in oligosaccharide-bound N-propanoylneuraminic acid. N-Propanoylmannosamine, applied to endogenously hyposialylated subclones of the myeloid leukemia HL60 and of the B-cell lymphoma BJA-B, both deficient in UDP-N-macetylglucosamine 2-epimerase, is efficiently metabolized to CMP-N-propanoylneuraminic acid resulting in up to 85%, of glycoconjugate-associated sialic acids being unphysiological N-propanoyleuraminic acid. Thus, UDP-N-acetylglucosamine 2-epimerase-deficient cell lines provide an important experimental progress in engineering cells to display an almost homogeneous population of defined, structurally altered sialic acids. (C) 2001 Published by Elsevier Science BN. on behalf of the Federation of European Biochemical Societies.
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